Bulletin Samara State Agricultural AcademyBulletin Samara State Agricultural Academy1997-32252782-4225Samara State Agrarian University50938Research ArticleFEATURES OF REPARATIVE REGENERATION OF ACHILLES TENDON IN THE CONDITIONS OF AUTOLOGOUS TRANSPLANTATION OF CELL POOLSlesarenkoN. ADoctor ontogenesis of Biological academy Sciences,Professor,Head functional of the Department «Animal Anatomy and Histology named after Professor A. F. Klimov»slesarenko2009@yandex.ruZharikovA. M.Graduate Student of the Department «Animal Anatomy and Histology named after Professor A. F.Klimov»am.zharikov@gmail.comFSBEI HE Moscow State Academy of Veterinary Medicine characteristics and Biotechnology K. I. ScryabinFSBEI HE Moscow State Academy вof Veterinary Medicine characteristics and Biotechnology K. I.Scryabin1508202053465023112020Copyright © 2020, Slesarenko N.A., Zharikov A.M.2020The aim of the study is identification of features of Achilles tendon healing in the conditions of auto-transplantation of the cellular element, reflecting the regenerative activity of stromal-vascular fraction. Modern regenerative treatment methods for animal with tendon injuries involve stromal-vascular cells application of adipose tissue. They represent cell pool with high plasticity, proliferative activity, and ability to secrete multiple angiogenic factors. However, there is no data on morphological and morphometric regenerative equivalents of their use in case of tendon ligaments injury. Micro morphometrical indicators are presented that substantiate the effectiveness of using autologous cellular elements in case of Achilles tendon injury. The influence of stromal-vascular fraction from rat adipose tissue on regenerative process during healing of an induced tendon tissue defect was evaluated which lead to an increase in the thickness of bundles of the first and the second orders in the experimental group, significant differences in comparison with the control of the fibroblastic cell pool, and the number of blood capillaries of these animals. Based on the positive data of the autologous cell pool on the regenerative ability of the Achilles tendon, its practicability for veterinary medicine was scientifically justified. The experimental model of the study is a fancy rat (n=20). Achilles tendon was the research material. A complex methodological approach was used, including experimental modeling, micro morphological studies, micro morphometry, and statistical data processing. It was revealed that experimental animals injected with stromal-vascular fraction, micro morphometric transformations of connective tissue were detected, aimed at thickening of bundles of the first and second orders, the transition of fibroblasts to structured and ordered fibrocytes (tenocytes), acceleration of collagenogenesis and vascularization enhancement.tendonfibersfractionregenerationvascularizationfibrocytesсухожилиеволокнафракциярегенерацияваскуляризацияфиброциты[Берсенев, А. В. Клеточная трансплантология - история, современное состояние и перспективы / А. В. Берсенев // Клеточная трансплантология и тканевая инженерия. - 2005. - №1. - С. 32-33.][Веремеев, А. В. Cтромально-васкулярная фракция жировой ткани как альтернативный источник клеточного материала для регенеративной медицины / А. В. Веремеев, Р. Н. Болгарин, М. А. Петкова, Н. Кац, В. Г. Нестеренко // Гены & клетки. - 2016. - Т. XI, №1. - С. 35-42.][Деев, Р. В. Профессор Александр Александрович Максимов: эволюция идей / Р. В. Деев // Гены & клетки. - 2014. - Т. IX, № 2. - С. 2-14.][Иванов, А. В. Современные представления о механизмах репаративной регенерации ахиллова сухожилия после его разрыва / А. В. Иванов, Д. В. Козлов // Вестник Смоленской государственной медицинской академии - 2015. - №4 - С. 74-79.][Ярыгин, В. Н. Тканевые клеточные системы - основа биомедицинских клеточных технологий нового поколения: контуры идеологии / В. Н. Ярыгин // Вестник РАМН. - 2004. - №9. - С. 12-19.][Glazebrook, M. A. Histological Analysis of Achilles Tendons in an Overuse Rat Model / M. A. Glazebrook, J. R. Wright, Jr. M. Langman, D. W. Stanish, J. M. Lee // Journal of orthopaedic research. - 2008. - P. 840-846.][Smith, R. K. Mesenchymal stem cell therapy for equine tendinopathy / R. K. Smith // Disabil Rehabil. - 2008. - №30. - P.1752-1758.]