Bulletin Samara State Agricultural AcademyBulletin Samara State Agricultural Academy1997-32252782-4225Samara State Agrarian University50950Research ArticleALFALFA HAYLAGE BIOCHEMICAL INDICATORS WITH BIOLOGICAL PRESERVATIVES IN DIFFERENT STORAGE TIMEFattakhovaZ. F.Candidate of Biological Sciences, Senior Researcher of the Department Agrobiological Researchfattahova.zf@mail.ruShakirovSh. K.Doctor of Agricultural Sciences, Professor, Chief Researcher of the Department Agrobiological Researchintechkorm@mail.ruSharafutdinovG. S.Doctor of Agricultural Sciences, Professor of the Department «Biotechnology, Livestock and Chemistry»gazimsharaf_kgay@mail.ruKhakimovI. N.Doctor of agricultural Sciences, Professor of the Department «Zootechnia»xakimov_2@mail.ruTatar Research Institute of Agriculture - a separate structural division of the Federal Research Center «Kazan Scientific Center of the Russian Academy of Sciences»FSBEI HE Kazan State Agrarian UniversityFSBEI HE Samara State Agrarian University1511202054606623112020Copyright © 2020, Fattakhova Z.F., Shakirov S.K., Sharafutdinov G.S., Khakimov I.N.2020The aim of the research is improving the biochemical properties and alfalfa haylage quality using biological preservatives. Feed from perennial grasses play an important role for cows. The feed value of alfalfa directly depends on the vegetation phase, conservation and processing method. Research of preservative production, as well as selection of the best, is relevant due to widespread use of silage and haylage for cattle feeding andits high usein the animal diet. The object of the study is the dynamics of the pH level and the accumulation of organic acids in alfalfa haylage, including alfalfa herbage of the Aislu variety. The experiment was conducted in the laboratory of the Department of agro-biological research of the Tatar Research Institute of Agriculture - a separate structural division of the Federal research center «Kazan scientific center of the Russian Academy of Sciences» in 2018. As a result of the experiments, it was found that the use of SIL-All 4×4 and Ferbak-SIL agents led to rapid acidification of the feed reaching pH = 4.7 already on the 10th day of storage, which served as an intermediate preserving factor that limited the intensity of the development of butyrate bacteria at the first stage of haylage and, future, provided good preservation of the feed. On the 60th day of storage, a significant difference (p≤0.05)with biological agents of SIL-All 4×4 and Ferbak-SIL taking into account the sum of three acids and mass of lactic and butyric acids, respectively, was found in haylage amounting to 1.13; 1.23; 0.22 % and 0.99; 1.25; 0.22 % relative to the control sample without the introduction of a preservative.haylagealfalfastoragepreservativeacidityindicatorstorageсенажлюцернахранениеконсерванткислотностьпоказатель[Буряков, Н. П. Влияние биологических консервантов на кислотность и энергетическую ценность люцернового сенажа / Н. П. Буряков, А. В. Косолапов, П. И. Мезенцев // Главный зоотехник. - 2018. - №6. - С. 24-29.][Пат. 2638188 Российская Федерация, МПК А 23 К 30/18. Способ силосования трав биологическим консервантом «Фербак-Сил Б-1» / Гибадуллина Ф. С., Шакиров Ш. К., Ибатуллина Р. П. [и др.] ; заявитель и патентообладатель ФГБНУ «ТатНИИСХ». - №2638188/13 ; заявл. 23.10.14 ; опубл. 12.12.17, Бюл. №14. - 13 с.][Победнов, Ю. А. Биологические особенности и принципы консервирования люцерны / Ю. А. Победнов, В. П. Клименко, А. А. Мамаев [и др.] // Достижения науки и техники АПК. - 2018. - Т. 32, №2. - С. 44-47. - DOI: 10.24411/0235-2451-2018-10211.][Фаттахова, З. Ф. Динамика изменения питательности сенажа люцернового по срокам хранения при применении различных биологических консервантов / З. Ф. Фаттахова, Ш. К. Шакиров, И. Т. Бикчантаев // Вестник Казанского государственного аграрного университета. - 2019. - Т. 14, №3. - С. 77-81. - DOI: 10.12737/article_5db95f99849449.22933527.][Bai, J. Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of alfalfa silage / J. Bai, D. Xu, D. Xie [et al.] // Bioresource Technology. - 2020. - №315(123881). - DOI: org/10.1016/j.biortech.2020.123881.][Guo, G. Fermentation quality andin vitrodigestibility offirst and secondcut alfalfa (Medicago sativa L.) silages harvested at three stages ofmaturity / G. Guo, C. Shen, Q. Liu [et al.] // Animal Feed Science and Technology. - 2019. - No 257(114274). - DOI: 10.1016/j.anifeedsci.2019.114274.][Lara, E. C. Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum / E. C. Lara, F. C. Basso, F. B. De Assis [et al.] // Animal Production Science. - 2016. - Vol. 56, №11. - P. 1867-1874. - DOI: 10.1071/AN14686.]