The purpose of the research is the theoretical substantiation of the development of methods for correcting violations of generative and endocrine function of the testes of offspring. Female white mongrel laboratory mice weighing 19-21 g were used as experimental animals. The animals were divided into 2 groups: intact and experimental. At the gestation stage of E11.5 pregnancy, intramuscular single injection of a 2% oil solution of the synthetic analogue of estrogen synestrol was performed at the same time of day. The name of the experimental group C-25 is formed taking into account the injected substance and its dose, synestrol 25 mcg/kg, the intact group was not exposed. In the course of research, it was revealed that when the synthetic analogue of estrogen synestrol is introduced into the mother's body, morphological changes in the structures of the testes of the offspring are observed. The results of morphometric analysis of the testes of the offspring of white mongrel laboratory mice with prenatal single administration of the synthetic analogue of estrogen synestrol at a dose of 25 mcg/kg showed changes in the structure of the organ, manifested in the form of: a decrease in the average number of Sertoli cells in C-25 18.4±1.1 (P≤ 0.05), compared with the intact group (20.8±1.9); reduction of the average number of spermatozoa in C-25 175.0±3.8 (P≤0.05), compared with the intact group (196.6±5.3). There was a change in the endocrine function of the testes of the offspring, expressed in a decrease in the average area of the nuclei of Leydig cells, in the intact group the indicator was 6.72±1.78, in the experimental group C-25 – 5.88±1.43 (P≤ 0.05). Exposure to a dose of synestrol at the E11.5 stage of the prenatal period of offspring development leads to persistent morphological changes in the testes of offspring in the postnatal reproductive period of ontogenesis.