Seasonal changes in anthropogenic microbial communities of digistate and silage

The method of gravitational mass spectroscopy (GMS) has been used to study the long-range order (LRO) in a complex of atomic nucleus clusters (ANC) in the range from 200 Da to 3.6 billion Da in washout rain waters of digistate, silage and their mixtures. Signals from genomes of a wide range of bacteria, viruses, fungal mitDNA, DNASE1, polymerases, and proteases were detected in the samples, the energy fraction of which in the ensemble and conformation of ANC changed as the samples aged from May to November 2024. The change in the structure of microbial communities occurred as a struggle for a water resource, when restructuring processes were constantly underway for the minimum potential energy of each ANC in the complex. The capsids of viruses were the first to be destroyed by proteases, then the membranes of bacteria, but not all. The mushrooms were more resistant. Bacterial genomes in the form of clusters became inactive. The genomes of capsid-free viruses selectively adsorbed proteins, fragments of the decay of their own capsids. One-hour sterilization by boiling the digistat wash, bringing the ethanol concentration to 50%, preserved only mushrooms (Fusarium sp.). A model of seasonal dynamics of anthropogenic microbial communities is proposed. The danger of anthropogenic microbial communities entering the environment is emphasized.

anthropogenic microbial communities, digistat, silage, bacteria, viruses, fungi, enzymes, water, long-range order, gravitational mass spectroscopy