Monitoring of Klebsiella pneumoniae as one of the criteria of microecological characterization of a water body

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Introduction. Representatives of the family Enterobacteriaceae are ubiquitous in the environment, causing severe, including opportunistic diseases in immunocompromised individuals. The occurrence of antibiotic-sensitive bacteria of the Klebsiella genus within the recreational zone of a water body is potent of posing an epidemiologic danger to the inhabitants of the settlement.

Materials and Methods. Water samples from the surface aquifers of the Don and Temernik rivers of Rostov-on-Don served as the material for the study. The sensitivity of isolates of Kl. pneumoniae isolates to fourteen antibacterial preparations were investigated by disk-diffusion method in accordance with the requirements of MUK 4.2.1980-04 and EUCAST v. 13.0 (2023). 13.0 (2023). Carbapenemase genes VIM, IMP, NDM, KPC, OXA-48, NDM were determined using commercial diagnostic kits AmpliSense MDR MBL-FL, AmpliSense MDR KPC/OXA-48-FL, AmpliSense MDR Ab-OXA-FL.

Results. In 2022, 247 isolates of Klebsiella pneumoniae were isolated in 14 biotopes of water bodies of Rostov-on-Don. Biotopes of epidemiological significance were identified with the presence of antibiotic resistance genes in 28 isolates isolated above and below the sewage discharge site (sampling point #1 and #5) and strains isolated from the mouth of the Temernik River (sampling point #14). Phenotype of multidrug-resistant (MDR) in point No. 1 possessed 22%, point No. 5 — 26.9%, point No. 14 — 26%; phenotype of extreme drug resistance (XDR) in point No. 1 possessed 11.1 %, point No. 5 — 15.3%, point No. 14 — 13%; phenotype of pan-drug-resistant (PDR) in point No. 1 did not possess, point No. 5 — 3.8%, point No. 14 — 4.3%.

Limitations. A limitation of the study is related to the location of water sampling from the river. Don and R. Temernik on the territory of Rostov-on-Don, covering from March to November 2022. In this article, we limited ourselves to sanitary-bacteriological and molecular genetic analysis.

Conclusion. The detection of PDR, XDR, and MDR antibiotic-resistant phenotypes of Klebsiella pneumoniae, as well as the detection of resistance genes, indicates to an increased risk of water-associated acute intestinal infections, especially in immunosuppressed individuals.

Compliance with ethical standards. The study does not require the submission of the conclusion of the biomedical ethics committee.

Contribution:
Kalyuzhin A.С. — Development of the study design, analysis of the obtained data, preparation of the manuscript;
Bayrakova A.L. — study design development, analysis of the obtained data, manuscript preparation;
Morozova M.A. — analysis of the obtained data, preparation of the manuscript, editing of the manuscript;
Latyshevskaya N.I. — editing of the manuscript;
Ruzhentsova T.A. — editing of the manuscript text.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: November 28, 2023 / Revised: February 20, 2024 / Accepted: March 11 / Published: April 10, 2024

作者简介

Alexander Kalyuzhin

Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing; Volgograd State of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing Medical University” of the Ministry of Health of Russia

编辑信件的主要联系方式.
Email: o.t.t.o.94@mail.ru
ORCID iD: 0000-0002-7234-6890

3rd year graduate student of the Department of general hygiene and ecology of the Volgograd State Medical University of the Ministry of Health of Russia, Volgograd, 400131, Russian Federation; junior researcher of the Dept. of Water Hygiene Institute of Complex Hygiene Problems of the Federal Scientific Center of Hygiene named after F.F. Erisman, Rospotrebnadzor, Mytishchi, 141014, Russian Federation

e-mail: o.t.t.o.94@mail.ru

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Alexandra Bayrakova

Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: alexandrabl@mail.ru

MD, PhD, senior researcher of the clinical microbiology and biotechnology laboratory of the Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky, Rospotrebnadzor, Moscow, 125212, Russian Federation; assistant at the Russian University of Medicine

e-mail: alexandrabl@mail.ru

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Marina Morozova

Rostov Research Institute of Microbiology and Parasitology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: morozova.q@mail.ru
ORCID iD: 0000-0003-2017-9717

MD, PhD, senior researcher of the Laboratory of Sanitary Microbiology of Water Bodies and Human Microbial Ecology of the Rostov Research Institute of Microbiology and Parasitology, Rospotrebnadzor; Rostov-on-Don, 344003, Russian Federation

e-mail: morozova.q@mail.ru

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Natalya Latyshevskaya

Volgograd State of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing Medical University” of the Ministry of Health of Russia

Email: latyshnata@mail.ru
ORCID iD: 0000-0002-8367-745X

MD, PhD, DSci., Professor, Head of the Department of general hygiene and ecology of the Volgograd State Medical University of the Ministry of Health of Russia, Volgograd, 400131, Russian Federation

e-mail: latyshnata@mail.ru

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Tatyana Ruzhentsova

Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: ruzhencova@gmail.com
ORCID iD: 0000-0002-6945-2019

MD, PhD, DSci., Professor, Deputy Director for the Clinical Work for Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky, Rospotrebnadzor, Moscow, 125212, Russian Federation

e-mail: ruzhencova@gmail.com

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