Histomorphometric study of rat liver during the treatment of the acute toxic injury

Introduction. There are a few effective therapies are available for acute liver injury at present. 

The aim of the study was to investigate histological and morphometric changes in the liver using models of toxic damage caused by carbon tetrachloride (ССl₄ ) and acetaminophen during correction with Oxymethyluracil (OMU).

Material and methods. A total of ninety rats were divided into 18 groups. The treatment of acute liver damage models caused by a single injection of ССl₄ or acetaminophen was carried out using “Heptor”, “Mexidol”, and OMU. The correction was carried out twice (sacrificed 24 hours after intoxication) and four times (sacrificed 72 hours after intoxication). Liver tissues were processed using standard histological techniques (H&E). A semi-quantitative assessment was performed using a scale based on the severity of liver cell deaths. 

Results. Twenty-four hours after administration of ССl₄ or 72 hours after administration of acetaminophen, the treatment with OMU led to a decrease in liver cell death compared to the group with administration of only ССl₄ or acetaminophen. Seventy-two hours after ССl₄ and 24 hours after acetaminophen intoxication, these groups with the OMU treatment did not differ from those of the carbon tetrachloride- or acetaminophen-induced liver injury groups, respectively.

Conclusion. Thus, on the model of ССl₄ liver injury, the treatment with OMU is more effective for 24 hours. In the case of acetaminophen intoxication, the effectiveness of treatment with OMU is better for 72 hours. The results obtained are possibly associated with a different mechanism of the damaging effect of the studied toxicants.

Contribution:
Baygildin S.S. — collection and processing of material, statistical processing, writing a text;
Repina E.F. — the concept and design of the study, editing;
Gimadieva A.R., Mustafin A.G. — oxymethyl uracil synthesis;
Kudoyarov E.R. — collection and processing of material, statistical processing;
Smolyankin D.A., Valova Y.V. — collection and processing of material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The work was carried out at the expense of subsidies for the implementation of a state task within the framework of the sectoral research program of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing (Rospotrebnadzor): “Hygienic scientific substantiation of minimizing risks to the health of the population of Russia” for 2016-2020 on topic 3.5, the state no. registration AAAA-A16-116022610045-4. The 5-hydroxy-6-methyl uracil composition was synthesized under the Ufa Institute of Chemistry of the Ufa Federal Research Center of the Russian Academy of Sciences (State Registration No. AAAA-A19-119011790021-4).

Conclusion of the bioethical commission: the study was approved by the bioethical commission of the Ufa Research Institute of Occupational Medicine and Human Ecology, carried out under the European Convention for the Protection of Vertebrate Animals Used for Experiments or Other Scientific Purposes (ETS No. 123), the directive of the European Parliament and the Council of the European Union 2010/63 / EC of September 22, 10 on the protection of animals used for scientific purposes.

Received: July 19, 2021 / Accepted: September 28, 2021 / Published: November 30, 2021