Gas chromatography/tandem mass spectrometry with long-term alkaline transesterification for the determination of esters of monochloropropanediols and glycidol with fatty acids in edible vegetable oils and fats
- Авторлар: Makarenko M.A.1, Malinkin A.D.1, Bessonov V.V.1
-
Мекемелер:
- Federal Research Centre of Nutrition and Biotechnology
- Шығарылым: Том 100, № 6 (2021)
- Беттер: 640-646
- Бөлім: METHODS OF HYGIENIC AND EXPERIMENTAL INVESTIGATIONS
- ##submission.datePublished##: 14.07.2021
- URL: https://bulletin.ssaa.ru/0016-9900/article/view/639311
- DOI: https://doi.org/10.47470/0016-9900-2021-100-6-640-646
- ID: 639311
Дәйексөз келтіру
Толық мәтін
Аннотация
Introduction. The article discusses the method of determination of 2-, 3-monochloropropanediol (2-MCPDE, 3-MCPDE) and glycidyl (GE) fatty acids esters in edible vegetable oils and fats by slow alkaline transesterification and GC-MS/MS. Many toxicological data showed these substances to have the potential for human health risks if consumed; in addition, existing determination methods have various limitations. So, there is a need to expand the variety of 2-MCPDE, 3-MCPDE and GE determination methods.
Materials and methods. 2- and 3-MCPD esters, and glycidyl esters in their corresponding free forms were measured by slow alkaline transesterification with GC-MS/MS.
Results. Multiple reactions monitoring mode parameters were optimized. Compared to SIM operating mode, MRM mode improved the selectivity and increased the signal-to-noise ratio. The method was linear in the range of 0.3 mg/kg - 30.0 mg/kg for each analyte. Recovery, tested in this range, was found to be 87.5%-113.6 %.
Conclusion. The method discussed here was based on the ISO 18363-2: 2018 standard. In contrast to the last one, we used tandem chromatography-mass spectrometry to detect analytes and a method of absolute calibration with internal standards to quantitate them. In addition, it is proposed to analyze a spiked sample during the sequence of unknown samples to improve the quality of results.
Авторлар туралы
Mariia Makarenko
Federal Research Centre of Nutrition and Biotechnology
Хат алмасуға жауапты Автор.
Email: dragon.soul1992@ya.ru
ORCID iD: 0000-0003-1688-6304
MD, junior researcher in the laboratory of food chemistry, Federal Research Center of Nutrition and Biotechnology, Moscow,
109240, Russian Federation.
e-mail: dragon.soul1992@ya.ru
РесейAlexey Malinkin
Federal Research Centre of Nutrition and Biotechnology
Email: noemail@neicon.ru
ORCID iD: 0000-0002-0370-4500
Ресей
Vladimir Bessonov
Federal Research Centre of Nutrition and Biotechnology
Email: noemail@neicon.ru
ORCID iD: 0000-0002-3587-5347
Ресей
Әдебиет тізімі
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