The GluN3A Subunit Forms GluN3A-NMDAR and the Excitatory Glycine Receptor in the Olfactory Sensory Neurons of Adult Wistar Rats

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Abstract

The article presents experimental data obtained on isolated olfactory sensory neurons (OSN) of adult Wistar rats. The aim of the study was to show by in vivo fluorescent confocal microscopy using pharmacological analysis whether the GluN3A-subunit is localized in the olfactory sensory neurons of adult animals. Analysis of the results have revealed the presence of GluN3A-subunit in the OSN of adult rats as part of the excitatory glycine receptor and GluN3A containing N-methyl-D-aspartate receptor (GluN3A-NMDAR). In addition to them, the typical NMDAR and non-NMDAR are localized in the OSN. All these receptors can be located both in the same and in different receptor cells, which manifests the heterogeneity of the OSN in the olfactory epithelium.

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About the authors

E. V. Bigday

Saint Petersburg State Pediatric University

Author for correspondence.
Email: bigday50@mail.ru
Russian Federation, Saint Petersburg

A. A. Zuykova

Saint Petersburg State Pediatric University

Email: bigday50@mail.ru
Russian Federation, Saint Petersburg

A. M. Barmasova

Saint Petersburg State Pediatric University

Email: bigday50@mail.ru
Russian Federation, Saint Petersburg

A. A. Razinova

Saint Petersburg State Pediatric University

Email: bigday50@mail.ru
Russian Federation, Saint Petersburg

A. V. Pozdnyakov

Saint Petersburg State Pediatric University

Email: bigday50@mail.ru
Russian Federation, Saint Petersburg

D. O. Ivanov

Saint Petersburg State Pediatric University

Email: bigday50@mail.ru
Russian Federation, Saint Petersburg

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2. Fig. 1. Examples of individual DOS responses to the applied stimuli, as well as confidence interval bar charts to illustrate DOS population responses. (a) - Effect of 3 μM glycine solution, 1 mM MgSO4 solution on the change in calcium fluorescence intensity in adult rat OSNs (arrow indicates onset of stimulation) (n = 46). (b) - Effect of 3 μM glycine solution, 1 mM MgSO4 solution and 100 μM NMDA solution on the fluorescence signal in rat OSNs (n = 30). (c) - Effect of 3 μM glycine solution, 1 mM MgSO4 solution and 100 μM NMDA solution on fluorescent signal in rat OSNs (n = 16). (d) - Effect of 3 μM glycine solution and 100 μM NMDA solution on the fluorescent signal in rat OSNs (n = 37). (e) - Effect of 3 μM glycine solution, 100 μM NMDA solution and 100 μM glutamate solution on fluorescent signal in rat OSNs (n = 29).

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3. Fig. 2. Heterogeneous distribution of NMDARs and non-NMDARs in olfactory sensory neurons of adult Wistar rats.

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